Sperm Chromatin Structure Assay: Its Clinical Use for Detecting Sperm DNA Fragmentation in Male Infertility and Comparisons With Other Techniques

Clinical aspects of sperm DNA fragmentation detection and male infertility.

Over the past 25 years, various methods have been developed to measure sperm DNA strand breaks in situ. Currently, there are four major tests of sperm DNA fragmentation, including the Comet, Tunel, sperm chromatin structure assay (SCSA) and the acridine orange test (AOT). The Comet assay is a light microscope technique where the sperm cells are mixed with melted agarose and then placed on a gla...

Environmental toxicants cause sperm DNA fragmentation as detected by the Sperm Chromatin Structure Assay (SCSA).

Studies over the past two decades have clearly shown that reproductive toxicants cause sperm DNA fragmentation. This DNA fragmentation can usually be detected prior to observing alterations of metaphase chromosomes in embryos. Thus, Sperm Chromatin Structure Assay (SCSA)-detected DNA damage is viewed as the molecular precursor to later gross chromosome damage observed under the light microscope...

An evidence-based perspective on the role of sperm chromatin integrity and sperm DNA fragmentation testing in male infertility

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We read with interest the commentary by Dr. Pandiyan and colleagues (1) about the merit of our recently published clinical practice guidelines for sperm DNA fragmentation (SDF) testing based on clinical scenarios (2). We noted that these authors were quite skeptic about the integration of SDF testing into the clinical...

Implications of ejaculatory sperm DNA fragmentation on male infertility management

Clinical Factors Associated with Sperm DNA Fragmentation in Male Patients with Infertility

OBJECTIVE The clinical factors associated with sperm DNA fragmentation (SDF) were investigated in male patients with infertility. MATERIALS AND METHODS Fifty-four ejaculates from infertile Japanese males were used. Thirty-three and twenty-one were from the patients with varicoceles and idiopathic causes of infertility, respectively. We performed blood tests, including the serum sex hormone le...